Mesenchymal stem cell extracellular vesicle production serum-free culture medium (item number: C04.004.002) is a specially designed extracellular vesicle production medium by Tongteng Xinchuang for human mesenchymal stem cells (including umbilical cord mesenchymal, bone marrow mesenchymal, adipose derived mesenchymal and other stem cells).
This product can be supplemented with a certain proportion of nutritional additives and prepared into a complete culture medium for use. It supports MSC (mesenchymal stem cells, abbreviated as MSC) 2D and 3D cell culture for harvesting extracellular vesicles.
Serum free culture medium for extracellular vesicle production of mesenchymal stem cells is a type of culture medium used to increase the production of extracellular vesicles in stem cells. Compared with similar domestic culture media, the extracellular vesicle production can be increased by more than 50%. And it can greatly reduce batch differences, lower the risk of various viruses, mycoplasma and other contamination.
Extracellular vesicle production
1. Cell recovery and seed expansion: After cell recovery, the original complete culture medium (recommended to use the mesenchymal stem cell serum-free basic culture medium kit, item number: C04.004.003) is used for culture and expansion until the exosome production stage.
2. During the cell expansion stage of MSC extracellular vesicle production, mesenchymal stem cell extracellular vesicles are used to produce serum-free culture medium for complete cell expansion.
3. During the MSC extracellular vesicle production and harvesting stage, that is, after 3-4 days of cell growth in complete culture medium, it is possible to independently choose whether to add nutritional additives to the culture medium according to process requirements.
4. If you choose to use complete culture medium, you can directly harvest the supernatant.
5. If choosing not to add nutritional supplements, rinse the cells with 1X PBS solution;
6. Then add mesenchymal stem cell exosomes to produce serum-free culture medium and continue culturing for a period of time. Collect the cell culture supernatant, which can be used for exosome extraction.
Recommended Use
“*”：In 3D cultivation mode (recommended to use CEL-G in combination) ® Culture Ad60 During the harvesting stage of extracellular vesicle production in a fixed bed bioreactor, the culture medium can be changed every 2-4 days under the premise of good cell condition, and multiple harvests can be achieved after changing the medium.